Adding N-terminal and C-terminal epitope tags optimizes full length protein purification


Posted May 14, 2014 by lorrainegenscript

In a recent scientific video journal, a group of scientists demonstrated a double-tagging affinity purification technique that allowed the recovery of highly purified, full length target protein.

 
In a recent scientific video journal, a group of scientists demonstrated a double-tagging affinity purification technique that allowed the recovery of highly purified, full length target protein. By adding an N-terminal GST and a C-terminal 10x His tag to their target protein, they eliminated degraded protein and were able to purify enriched, full length target protein successfully.


The use of epitope tags eliminates the need to use a different antibody for each newly purified protein.

GenScript offers more than 90 highly specific monoclonal and polyclonal antibodies against more than 40 different tags. Our tagged antibodies go through extensive quality control to ensure optimal sensitivity and consistent high performance.
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Issued By lorraine xu
Website Adding N-terminal and C-terminal epitope tags optimizes full length protein purification
Country United States
Categories Health
Tags genscript USA Inc , antibody products
Last Updated May 14, 2014