NGS Next Generation Sequencing, Advancement In Biotechnology


Posted March 17, 2015 by Briandiesel2

With its unprecedented throughput, scalability, and speed, NGS next-generation sequencing enables a researcher to study biological systems at a level that has never been possible before.

 
NGS Next-generation sequencing, makes large-scale whole-genome sequencing accessible and practical for the average researcher. NGS makes sequence-based gene expression analysis a “digital” alternative to analog techniques. It lets one quantify RNA expression with the breadth of a microarray and the resolution of qPCR. Microarray gene expression measurement is limited by noise at the low end and signal saturation at the high end. In contrast, next-generation sequencing quantifies discrete, digital sequencing read counts, offering an unlimited dynamic range. NGS is highly scalable, allowing researchers to tune the level of resolution to meet specific experimental needs. Targeted sequencing allows researchers to focus their research on particular regions of the genome. Choose whether to do a shallow scan across multiple samples, or sequence at greater depth with fewer samples to find rare variants in a given region.

NGS next generation sequencing utilizes a fundamentally different approach from the classic Sanger chain-termination method. It leverages sequencing by synthesis (SBS) technology – tracking the addition of labelled nucleotides as the DNA chain is copied – in a massively parallel fashion. Next-generation sequencing generates masses of DNA sequence data that's richer and more complete than is imaginable with Sanger sequencing. Sequencing systems from CD Genomics can deliver data output ranging from 300 kilobases up to 1 terabase in a single run, depending on instrument type and configuration.

The high demand for low-cost sequencing has driven the development of high-throughput sequencing, which is also termed as NGS next generation sequencing .The best next generation sequencing will reach by more traditional wet-lab scientists. Lately, genome-wide scale procedure analysis is increasingly being used as a keystone to foster the discovery in biomedical research. DNA sequencing is done by the scientists in a very fine manner. In the first step the DNA must be extracted from the chromosome that keeps it, and then it is broken up into smaller strands. Each small form is used to make a set of parts; each one's base is shorter than the one before it. Later, the parts are separated by a gel and then a fluorescent dye is used to identify each individual type of nucleotide. Then a computer program generates the sequences of the nucleotides into the units of about 500 bases that just replicate the strand that the DNA was taken from. These units are then bent to make a very long strand of DNA sequencing. The resulting DNA sequencing is then shared with other scientists in computer databases.

Technology provides advanced multi-omics and bioinformatics service solutions for its global customers who do research in laboratory. CD genomics possesses significant contributions to genetic research. Equipped with the industry's broadest array of cutting-edge technologies and coupled with an experienced team of scientists and bioinformaticians. CD Genomics delivers rapid, cost-effective and high-quality NGS next generation sequencing solutions and results that enable researchers to achieve scientific breakthroughs.
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Tags aptamer , genetic health , genome , genotyping , sequencing
Last Updated March 17, 2015