We learned about PCR in high school biology, but what is PCR? Polymerase chain reaction (PCR) is a molecular biological technology used to amplify and amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. The greatest feature of PCR is that it can greatly increase the amount of DNA. So whatever bits of DNA can be isolated from fossils, the remains of historical figures, or the hair, skin or blood of a murder perpetrator decades ago, can be amplified and compared using PCR. This is where the power of "microevidence" comes in. Mullis of the United States first put forward the idea in 1983. In 1985, he invented polymerase chain reaction, namely simple DNA amplification method, which means the real birth of PCR technology. By 2013, PCR has developed into the third generation technology. In 1973, chia-yunqian, a scientist from Taiwan, discovered the stable Taq DNA polymerase, which also made a fundamental contribution to the development of PCR technology. PCR (polymerase chain reaction) is to use DNA in vitro degeneration becomes single 95 ° centigrade high temperature, low temperature (often about 60 ° C) primers with single combination according to the principle of complementary base pairing, adjustable temperature to the optimal reaction temperature DNA polymerase (around 72 ° C), DNA polymerase along to five carbon sugar phosphate (5 '3') in the direction of the complementary strand synthesis. PCR instrument based on polymerase is actually a temperature control device, which can be well controlled between denaturation temperature, renaturation temperature and extension temperature. and PCR has changed the course of modern biology, so we will talk about some important PCR in this paper.

1. LAM PCR

LAM PCR (Linear - amplification mediated PCR) is a technology which is used for identifying and characterizing unknown flanking DNA adjacent to known DNA of any origin. More specifically, LAM-PCR has been developed to localize viral vector integration sites (IS) within the host genome [1, 2]. Genetic elements like retroviruses or transposons integrate their genome into the host genome in a (semi-) random manner [3-6]. In many cases, it is decisive to know exactly the position where these vectors integrated. With years of development, Creative Biogene has set up mature assays for several vectors, including lentiviral and most oncoviral vectors, sleeping beauty transposons, and some adeno-associated Virus related vectors, etc.. The application of viral vectors in gene therapy is rapidly developing and the therapeutic results are highly promising. However, actively and passively integrating (viral) vectors may fail to deliver, or trigger severe side effects by undirected or unintended integration into the genome of the target cell. Thus, the analysis of vector integration sites in target cells is extremely important to address both biological and safety issues. Creative Biogene, as a leading biotechnology company in the world, has extensive expertise and experience which are available to provide you with customer LAM PCR service to analyze the viral vector insertion/integration site.

2. Direct pcr

Direct PCR is a technique to amplify the target fragment directly from various biological samples. It is usually used for high throughput screening, gene detection and genotyping. Compared to the normal PCR, direct PCR is faster and more convenient. In addition, this technique requires very little sample material, and usually has a high PCR success rate and reproducibility.Directpcr has a lot of kinds.Today I will introduce two of these.

3. Human tissue direct PCR

Human Tissue Direct PCR Kit is specifically designed for amplification of DNA from unpurified human samples including buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even Formalin-Fixed, Paraffin Embedded (FFPE) human tissue samples. No DNA purification is required and unpurified DNA can be directly used as template for PCR.

4. Cell direct PCR kit

The Cell Direct PCR Kit is designed to rapidly extract and amplify genomic DNA from cultured animal cells in 96-well plates or other plates. No DNA purification is required and unpurified DNA can be directly used as template for PCR within 20 minutes.

So much for the introduction of PCR in this article. I hope you can have a deeper understanding of PCR through this article