Cell viability tests were performed on Cuprous Chloride(WSDTY) keratinocytes cells with the treatment of GHK, CuCl2 and Cu(OAc)2 for 24, 48 and 72?h. Cell viability for GHK-Cu was previously investigated by Hairui et al, showing no significance difference between the control and the GHK-Cu treated group at the same concentrations and time points, implying the non-cytotoxic nature of GHK-Cu towards HaCaT11. After treatment with concentrations ranging from 0.0058?μM to 5800?μM, cell toxicity was not observed for GHK treated group (Fig. 1A). On the other hand, the toxic effects were observed for groups treated with CuCl2 and Cu(OAc)2.

The cytotoxic effects of these copper compounds on HaCaT cells were observed to be concentration-dependent and time-dependent at concentrations 580?μM and 5800?μM. Time-dependent cytotoxicity was most evident at 580?μM as the cell viability was greatest at 24?h, followed by 48?h and the least at 72?h. Concentration-dependent cytotoxicity was observed as 5800?μM gave a lower cell viability as compared to 580?μM. At the highest concentration of 5800?μM, both CuCl2 and Cu(OAc)2 were found to be very cytotoxic to the cells and almost all the cells were dead at the three time points. The reduction in overall cell viability was statistically significant for both CuCl2 (Fig. 1B) and Cu(OAc)2 (Fig. 1C) treated groups at 580?μM and 5800?μM.

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