This article explores the advanced techniques and applications of isolating dendritic cells, highlighting the use of pluriBead technology for efficient and gentle cell separation in immunological research.

Dendritic cells (DCs) are pivotal to the immune system, serving as key antigen-presenting cells that bridge the innate and adaptive immune responses. Their ability to process antigen material and present it on the cell surface to T cells makes them crucial for initiating and modulating immune responses. Understanding and isolating dendritic cells is vital for immunological research and therapeutic development. Advanced cell separation technology, including particle filtration and antibody cell separation, plays a significant role in the efficient isolation and enrichment of dendritic cells for various applications.

Advanced Cell Separation Technology for dendritic cell separation

Cell separation technology is critical for isolating specific cell populations from complex biological samples. This technology encompasses various methods, including particle filtration and antibody cell separation, which are essential for obtaining pure and viable dendritic cell populations for research.

Particle Filtration and Antibody Cell Separation

Particle filtration involves the use of filters to separate cells based on size and density. This method ensures that unwanted debris and contaminants are removed, providing a cleaner sample for further processing. Antibody cell separation, on the other hand, utilizes specific antibodies to target and isolate desired cell populations. These methods are often combined to achieve high purity and yield of dendritic cells.

pluriBead Technology for Dendritic Cell Isolation

pluriBead offers a unique and efficient cell separation technology that works without any magnetic components. This innovative method involves sieving pluriBeads with bound target cells through a cell strainer. The pluriBeads with the target cells remain on top, while unwanted cells pass through. After detaching the target cells from the beads, researchers have their desired cell population ready for use.

CD11c pluriBead: Specific Isolation of Murine Dendritic Cells

For isolating dendritic cells from murine samples, the CD11c pluriBead suspension is an excellent tool. This product is specifically designed for the positive isolation of CD11c+ target cells from any murine sample material, providing researchers with a reliable method for obtaining high-purity dendritic cells.

Key Features of pluriBead Technology

No Sample Preparation: pluriBead technology simplifies the process by allowing the direct use of samples ranging from 200 µl to 45 ml without the need for density gradient centrifugation or erythrolysis.

Versatile Sample Material: This technology is compatible with various sample materials, including whole blood, buffy coat, PBMC, secretion/excretion material, brain homogenate, spleen, liver, and more.

Wide Range of Species: pluriBead technology supports the isolation of cells from multiple species, including human, mouse, rat, bovine, canine, sheep, and others.

Fast Isolation: The isolation process can start from as little as 5 minutes, making it highly efficient for researchers with time-sensitive projects.

Gentle Isolation: High yield of viable cells minimizes sample requirement, ensuring that the integrity and functionality of the isolated cells are maintained.

Universal pluriBeads: These beads can be used with any external antibody, allowing for customizable cell separation strategies tailored to specific research needs.

Simultaneous Cell Isolation with pluriBead Cascade: This feature enables the separation of two different cell types at the same time from one sample material, increasing efficiency.

Sequential Cell Isolation: Researchers can isolate up to six different targets from one sample, providing versatility in experimental design.

Conclusion

Dendritic cells are essential for initiating and regulating immune responses, making their isolation and study crucial for advancing immunological research and developing targeted therapies. pluriBead’s advanced cell separation technology, including the CD11c pluriBead for murine samples, offers researchers an efficient and reliable method for isolating high-purity dendritic cells. With its versatility, ease of use, and high yield, pluriBead technology is a valuable tool for scientists seeking to explore the complexities of the immune system and drive forward the field of immunology.

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