All unwanted cells are eliminated by negative cell selection. In contrast to positive cell enrichment, specific antibodies will bind to and separate all cells except the cells of interest. The desired cells are left unbound and "untouched" by antibodies or beads in the sample material while the undesirable cells are eliminated. The pluriSpin system isolates live, unaltered, and highly purified cells in a single step without the use of magnets or columns. As a result, there is less chance of the target cells becoming activated or damaged.

Advantages of Plurispin

The stress of being passed through a column is not applied to the cells. The desired cells lack antibodies and contain no beads. This prevents unintentional activation or blocking of the population of interested cells. All undesirable cells are removed through negative cell selection. All cells, excluding the cells of interest, will be bound to particular antibodies and separated, in contrast to positive cell enrichment. The desired cells are left "untouched" by antibodies or beads in the sample material, while the undesirable cells are eliminated.

Without using magnets or columns, the pluriSpin system isolates live, unaltered, and highly purified cells in a single step. As a result, there is a lower chance of activation or damage to the targeted cells. No specialized knowledge or tools, such as specialized instruments or magnets, are needed for PluriSpin. It only needs a mixer, like our magnetic stirrer adapter or a whipping rolling mixer (pluriPlix).

Facts & Features of PluriSpin
inexpensive negative cell separation
Based on density centrifugation and independent of RBC concentration
Maximum viability requires unaltered cells.
Without columns or magnets
PLT Depletion can be used to remove platelet contamination from whole blood, buffy coat, or cord blood.

Solution and advantages with pluriSpin Human PLT Depletion

• Using density gradient centrifugation increases the purity of enriched cells in a single step.
• Can be used in conjunction with negative separation techniques such as pluriSpin.
• Improved enrichment of rare cells (e.g. in use with pluriSpin CD45 Depletion)
• The blood sample undergoes the least amount of manipulation due to the shorter centrifugation step (compared to standard protocol with platelet density gradient)
• The enrichment of cells from blood samples older than 8 hours will be enhanced.

Let’s Tackle The Cell Separation Obstacles Easily
Our products provide a simple and elegant solution to a virtually limitless number of applications and industries. We're here to help with whatever challenges you're facing, from expanding diagnostic capabilities to rare cell and environmental applications.