Sanger sequencing is a technique that has been used for several decades to determine the nucleotide sequence of DNA. It is named after its inventor, Fred Sanger, who was awarded the Nobel Prize in Chemistry in 1980 for this breakthrough technology. Sanger sequencing remains a popular and widely used method for DNA sequencing, and companies like Genewiz offer Sanger sequencing services for researchers and scientists. In this comprehensive guide, we will explore the basics of Sanger sequencing and the services offered by Genewiz.

What is Sanger Sequencing?

Sanger sequencing is a method used to determine the nucleotide sequence of a DNA molecule. The method involves copying the target DNA sequence using a DNA polymerase enzyme and a primer. The primer is a short sequence of DNA that binds to the target sequence and initiates the copying process.

As the DNA polymerase enzyme adds nucleotides to the growing copy, it also incorporates fluorescently labelled nucleotides emitting a unique colour when a laser excites them. The coloured signals are detected and used to read the sequence of the DNA molecule.

What are the steps involved in Sanger sequencing?

Sanger sequencing involves several steps, including:

01. Template Preparation: The first step is isolating and preparing the DNA sequencing template.

02. PCR Amplification: The DNA template is amplified using PCR to generate enough copies for sequencing.

03. Purification: The amplified product is purified to remove any unwanted byproducts.

04. Sequencing: The purified product is then sequenced using the Sanger method.

05. Data Analysis: The sequence data is analyzed and assembled into a final sequence.

What are the advantages of Sanger sequencing?

Sanger sequencing offers several advantages over other sequencing methods, including:

01. High Accuracy: Sanger sequencing has a high accuracy rate and can detect single nucleotide changes.

02. Established Technology: Sanger sequencing has been widely used for decades and is a proven technology.

03. Cost-Effective: Sanger sequencing is more cost-effective than newer sequencing methods for small-scale projects.

Bottom Lines:

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