Learn about FACS Staining Protocol at Facs-Analysis.com


Posted September 13, 2019 by facsanalysis

Did you know that the process of DNA analysis is widely employed by forensic experts for the purpose of identification and DNA finger-printing.

 
Did you know that the process of DNA analysis is widely employed by forensic experts for the purpose of identification and DNA finger-printing. DNA testing and genetic analysis plays a critical role when confirming a person's identity. Genetic evidence has to be collected before the process of DNA testing can begin. Such evidence can be collected from one's saliva, blood, hair or semen samples. Once the DNA is extracted from the samples it is then isolated from the individual cells and be analysed utilizing various methods such as PCR and RFLP. The results of the analysis can thereafter be used for the initial and intended purpose of the DNA test. At Facs-Analysis.com you can learn everything about FACS staining protocol.

FACS Protocols

FACS Protocols
Direct immunostaining of surface antigens
Indirect immunostaining of surface antigens
General immuno-staining procedure for intracellular antigens
Intracellular cytokine/phospho-immunostaining
In Vitro Cell Stimulation Reference Table
Dye efflux staining
DNA content or Cell cycle analysis

FACS is an abbreviation for fluorescence-activated cell sorting, which is a flow cytometry technique that further adds a degree of functionality. By utilizing highly specific antibodies labelled with fluorescent conjugates, FACS analysis allows you to simultaneously collect data on, and sort a biological sample by a nearly limitless number of different parameters.

Just like in conventional flow cytometry, forward-scatter, side-scatter, and fluorescent signal data are collected. The user defines the parameters on how cells should be sorted and then the machine imposes an electrical charge on each cell so that cells will be sorted by charge (using electromagnets) into separate vessels upon exiting the flow chamber. The technology to physically sort a heterogeneous mixture of cells into different populations is useful for a wide range of scientific fields from research to clinical. Nowadays the terms “flow cytometry” and “FACS” are often used interchangeably to describe this laser-based biophysical technique.

About Facs-Analysis:

Facs-Analysis is the Flow Cytometry Technical Resource Center. You can learn everything about flow cytometry and get answers to questions like "Do you know the FACS Principle? What is flow cytometry, the Flow Cytometer, Fluorescence and Fluorophore (Fluorochrome)?

For more details please visit the website here: https://www.facs-analysis.com/.

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Tags Flow Cytometry System , General Experimental Procedure , FACS Protocols , FACS Staining Protocol , Intracellular Staining Protocol , Flow Cytometry Immunology , Cell Cycle Analysis , Flow Cytometry Antibody Staining Procedure
Last Updated September 13, 2019