Welcome To The Modern Way Of Experiment - ELISA


Posted December 5, 2018 by antibodyelisa

Antibody and ELISA Experts proudly provide all the information related to performing ELISA. We are basically involved in offering ELISA kits to the customers to be used in research area.

 
ELISA is not new when it comes to detecting and measuring total count of antibodies in the blood stream. This test is basically brought in use when any antibodies related infection is detected. The antibodies are released from the body in response of antigens.

ELISA Test Is Generally Performed To Diagnose

• HIV/AIDS
• Lyme disease.
• Syphilis
• Zika Virus
• Rotavirus
• Pernicious anemia
• Rocky mountain spotted fever
• Toxoplasmosis.

For your information, ELISA is often used as screening tool to do a deep analysis.

Performing The Test

Basically, the ELISA has very simple and straightforward mechanism. For performing the test, the doctor needs a sample of your blood. The whole process is relatively painless. According to Elisa Sample Preparation, the sample is generally placed to a Petri dish which already comprises of specific antigen which is used for doing testing. In case if the blood has the presence of antibody the reaction could easily be seen. The presence of antibody is mostly determined by the change of colour in the Petri dish.

Benefits Of ELISA Test

Till now, most of you have understood that ELISA is vital technology when it comes to immunoassay. Apart from diagnosing the diseases, it is also meant for performing quantitative assay of macromolecular antigens along with micro molecular antigens. The doctors also choose this test for performing sero-epidemiological investigation.

In true words, ELISA is expanding the scope of early diagnosis in biomedicine field.

Detecting Hepatitis C Virus Antibody Assay

As per Sandwich Elisa Principle, the HCV antibody assay requires double-antibody sandwich. The first step is to make reagent of double-antibody sandwich. Thereof, there is no requirement of diluted serum sample and own high specificity. At the second step, reagents are tested with all types of antibodies present in the serum. Due to this, the window period starting from the onset of virus infection to assaying of the antibody automatically shortens up.

Ideal For Lyme Disease

The lab technicians perform the experiments to check the sensitivity of ELISA when it comes to Lyme disease. If you do not know what exactly the Lyme disease is so let us tell you. It is known as a multisystem inflammatory disorder that generally shows wide spectrum of various skin infections. ELISA is one such test which is used for early detection of Lyme disease and helpful in preventing systematic complications.

Note Down The Benefits Of ELISA In Detecting This Disease

• In general, ELISA comprises of very sensitive antigens which are available.
• According to various diseases, B. burgdorferi is considered as the best due to its high specificity and sensitivity feature.

In A Nutshell

It is seem that the sensitivity of a test differed in different stages. Hence, it is completely proven that ELISA is suitable for complicated diseases.

Boster Antibody and ELISA Experts proudly provide all the information related to performing ELISA. We are basically involved in offering ELISA kits to the customers to be used in research area.
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Tags elisa plate coating protocol , elisa sample preparation , sandwich elisa principle , sandwich elisa troubleshooting
Last Updated December 5, 2018