Pipettes are a standard sight in any chemistry, biology or a physics lab . Popularly referred to as pipette, pipettor, chemical dropper or a pipet, it's a standard laboratory tool to transfer a measured volume of liquid. they're available in several designs for various purposes.

Micropipettes are those dispensing between 1 and 1000 μl and in contrast, macropipettes dispense greater volumes. Depending upon the sizes, capacity and elegance , pipettes is of following types:

• Air displacement micropipettes
• Positive displacement pipette
• Volumetric pipettes
• Graduated pipettes
• Pasteur pipette
• Transfer pipettes
• Van Slyke pipette
• Ostwald–Folin pipette
• Glass micropipette
• Extremely low volume pipettes
• Microfluidic pipette

But before employing a pipette, it's extremely necessary that you simply master their use so as to achieve success within the lab experiments.

Parts of Micropipette

a) Plunger button
b) Tip ejector button
c) Volume adjustment dial
d) Digital volume indicator
e) Shaft
f) Attachment point for a disposable tip

Sizes of micropipette

Basically, micropipettes are available three different sizes of varying capacity. they're P20, P200 and P1000. The sizes are noted on the highest of the plunger button (as mentioned earlier, part a)

Pipette Tips

You should always remember that liquids are never drawn directly into the shaft of the pipette. For this, a disposable plastic tips are attached to the shaft and for adjusting the quantity of the liquid, there's a black volume adjustment dial near the highest of the micropipette.

There are two sizes of tips. The larger blue tips are used for the P1000 and therefore the smaller clear tips are used for the P20 and P200. you ought to also lookout of not contaminating the ideas , for example: – close the box of the ideas (racked in), after the utilization in order that airborne contamination are often prevented.

How to insert a tip

Before inserting a tip, you ought to keep some essential points in mind as given below:-
• Select the right size tips.
• Open the box without touching the ideas together with your hands.
• Insert the micropipette shaft into the tip and depress firmly. this may attach the tip to the shaft.
• Remove the micropipette, with the tip attached.
• Close the box without touching the ideas together with your hands
• Remember, it's better to handle something carefully instead of doing it sort of a defaulter.