How to use Coronavirus (COVID-19) Testing to detect?


Posted July 3, 2020 by wanglei

COVID-19 is a respiratory illness caused by a new virus. Symptoms include fever, coughing, a sore throat and shortness of breath. The virus can spread from person to person, but good hygiene can prevent infection.

 
The kit uses colloidal gold immunochromatography. The chromatographic strip is mainly composed of a binding pad, a nitrocellulose membrane and absorbent paper attached to a plastic bottom plate. Among them, there is colloidal gold-labeled new coronavirus antigen and quality control protein on the binding pad; there are three coating lines on the nitrocellulose membrane, which are respectively coated with 1) anti-human IgM antibody (M line), 2) anti-human IgG antibody (G Line), 3) quality control antibody (line C). During the test, the sample and sample diluent are added dropwise to the binding pad. If there is anti-new coronavirus IgM and IgG in the sample, the new coronavirus antigen labeled wit colloidal gold on the binding pad is combined to form lg M-new coronavirus antigen.Colloidal gold complex (complex M) or/ and IgG-new coronavirus antigen-colloidal gold complex (complex G). The above complex is chromatographed with the nitrocellulose membrane toward the end of the absorbent paper. When passing through the M line, the complex M is captured by the anti-human IgM antibodyWhen the G line passes through the G line, the complex G is captured and enriched by the anti-human IgG antibody, so that the G line appears the purple signal. When the C line passes, the quality control protein labeled with colloidal gold is controlled by quality control. The antibody captures the C-line with a mauve signal.

1. Open the box and take out the test paper and diluent, and leave it at room temperature for 15-30 minutes
2. Pipette 20ul of whole blood or 10ul of serum and plasma with a sampler and add it to the sample well.
3. Add 2 drops of sample dilution to the well.
4. Wait for 10 minutes and observe the results, After 15 minutes, the observation results are invalid for reference only.
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Issued By wanglei
Country China
Categories Biotech , Blogging
Tags Testing for COVID-19 , COVID-19 Rapid Test , Coronavirus testing
Last Updated July 3, 2020