You can find IHC Antigen Retrieval Optimization Tips at the resource immunostaining.info. While selecting the right antigen retrieval method, the process of fixing a tissue sample with paraformaldehyde
When looking at antigen retrieval methods, techniques usually fall into two main categories, PIER (protease-induced epitope retrieval) and HIER (heat-induced epitope retrieval).
Tissue sectioning is an art. The selection of knife material, blade shape, cutting speed, knife angle and other variables must be determined through experience with the type of tissue and the particular equipment.
Tissue Sectioning at Immunostaining.info is Slide Pre-Treatment. The pre-treatment procedure is designed to prevent peeling caused by elevated temperature, high pressure, radiation and other factors.