Science: Imaging technology to monitor the spread of HIV in vivo
How the transcriptional viruses (such as HIV) spread in the host, scientists are not clear, recently, researchers from Yale University designed a method to observe the HIV diffusion process in living organisms. Related research was published in the international journal Science, in which researchers successfully observed how HIV reaches and spreads in mouse lymph nodes.
Researcher Professor Walther Mothes said that the way we observed the spread of the virus is not the same as people imagined. In the experiment, we tracked the fluorescently labeled virus in the mouse body and used complex imaging techniques to capture the combination of viral particles. The process of phagocytosis, which is accomplished by viscous proteins located on the surface of the lymph nodes.
The researchers said that the captured virus particles can be opened to a rare type of B cells, and then the virus particles will adsorb themselves to the tail of these B cells and be dragged into the lymph nodes. These B cells will be the same within one to two days. The organization establishes a stable connection to promote the complete transmission of the virus. The video taken by the researchers describes a potential way to help suppress the tissues surrounding the HIV-infected area. If researchers can develop a way to block HIV's use of sticky proteins to bind macrophages, then the virus Diffusion propagation is suppressed.
 Scientists develop the world's first next-generation sequencing technology to measure HIV drug tolerance mutations
At the ACCC Annual Meeting and Clinical Lab Expo, researchers from Singapore's genetic sequencing company, Vela Diagnostics, launched the world's first HIV drug resistance mutation. A new generation of sequencing technology that plays an important role in helping clinicians optimize HIV treatment systems, while also helping scientists to take the initiative to minimize the global antiretroviral drug tolerant epidemic.
The use of antiretroviral therapy for HIV infection has grown dramatically over the past decade and is part of the current global adoption of the AIDS public health threat program; according to data from the World Health Organization, HIV drug tolerance Concurrent growth will counteract the efforts of scientists over the years by offsetting the effects of antiretroviral drugs on HIV and AIDS progression; therefore, testing patients' tolerance to HIV drugs is key to ensuring patients receive effective treatment. At the same time, it is also very important to effectively manage the tolerance of antiretroviral drugs.
Analy Chem: A new mobile DNA detection technology for monitoring HIV
Recently, in a research report published in the international magazine Analytical Chemistry, scientists from the University of Rice in the United States have developed a simple and high-accuracy detection technology to detect the signs of HIV and the degree of disease development in patients' bodies.
Researcher Rebecca Richards-Kortum said that the current gold standard for diagnosing HIV status in infants relies on laboratory equipment and currently available detection techniques to monitor viral load in their bodies, and researchers in this paper have developed New HIV-based detection technology based on nucleic acids.
The new detection technology is based on a PCR-based method for amplification of recombinant polymerases (RPA antibody), which replaces complex laboratory testing steps that allow rapid multi-amplification of genetic markers in the blood, resulting in genetic markers of the virus. The object can be easily detected; the qRPA test can be used to perform targeted labeling of fluorescent probes on specific sequences in HIV DNA, thereby using a machine for quantitative detection, and finally by software analysis of fluorescent DNA, doctors can master Dynamic information about HIV in the patient's body.
 Brushing saliva for 15 minutes to test AIDS
The most common method of AIDS testing is to use the kit to detect and pump blood tests. Kit testing typically requires 50 or more people to work together, and the results typically take several days. The rapid blood test can get the test results within 30 minutes, but it requires professional doctors to operate around. So, is there a simpler and more practical detection method?
In the Technology Incubation Park in the High-tech Zone of Suzhou, China, Suzhou Wanmuchun Biotechnology Co., Ltd. revealed that they independently developed the first integrated HIV saliva detector in China and successfully obtained the highest level of production license for the national medical device. . “Efficient, it can be read in 10 to 15 minutes; it is convenient to brush saliva; protect privacy, patients can self-test at home.” The company's R&D personnel took a small test stick from the box and showed it to reporters, as long as Stretch the gums in the entrance cavity, and after the test stick is taken out of the mouth for a while, the “display window” will show colored lines, and the test results will be clear at a glance.
 British scientists have developed a fast and cheap HIV test method
Scientists at Imperial College of Science and Technology have developed a new HIV test that is 10 times more sensitive than current tests, but at a relatively low cost, which is a diagnosis for HIV-infected people in developing countries. Treatment brings a new gospel.
At present, the use of human saliva to detect HIV has been quite simple and fast, but its shortcoming is that it can only be detected when the viral load reaches a certain concentration. In some cases, the viral load of the test sample is too low, and it is difficult to obtain accurate results using some of the original detection methods (such as saliva detection), and a "false negative" test result usually occurs. The new method can accurately detect the situation where the viral load is too low.
The new detection method uses nanotechnology, which can display the detection result of the sample to be tested as red or blue, which can be distinguished by ordinary naked eyes. The method is to test the serum and detect in the disposable vessel whether there is an HIV biomarker called p24. If p24 is present, it will cause tiny gold nanoparticles to condense in an irregular manner, which in turn turns the solution blue; a negative result can separate the gold nanoparticles into a spherical shape. The solution is shown in red.
 American scientists developed molecular microscopy for in situ detection of HIV
The HIV in situ analysis technology has once again made a breakthrough. At the International AIDS Conference held last week, US scientists demonstrated their new detection technology and test results. This probe called "Molecular Microscope" can accurately detect the hidden places of HIV inside and outside the cell.
Ricardo Pul, deputy director of the Vaccine Research Center of the American Institute of Allergy and Infectious Diseases, said that the new technology of this molecular microscope is magical, and its super powers can fully understand the traces of HIV in any cell, and ultimately Help clear the mystery of the long-term survival of HIV and completely remove it from the body.