CD Genomic Company is able to provide the 2b-RAD technique, the upgraded RAD technique to simplify genome sequencing technique thereby achieving genome-wide high-throughput SNP genotyping.
The use of simplified genome sequencing technology for large-scale high-throughput SNP genotyping is currently a hotspot in the field of animal and plant genomics research. Simplified genome sequencing refers to the fragmentation of genomic DNA by restriction enzyme digestion, selection of some of the characteristic fragments, and the development and classification of high-throughput genetic markers within the genome range by second-generation sequencing technology.
According to Mike Jorge, a core researcher of 2b-RAD in CD Genomics, “The widely used technology is the RAD technology invented by the University of Oregon. However, RAD technology has two main technical defects. One is the Cumbersome database construction process, which involves multi-step DNA separation and purification process, which will have serious impact on technical repeatability and reliability; Besides, what RAD technology creates is a restricted DNA fragment of different lengths, and since PCR technology itself has the preference of amplification template length (that is, preferential amplification of small fragment DNA), it will seriously affect the expansion after the PCR and sequencing phase of emulsion PCR, increasing the genome representation of DNA fragments.
Considering the above drawbacks, CD Genomics comes up with 2b-RAD technology. The 2b-RAD technology effectively overcomes the inherent flaws of the above RAD technology. The 2b-RAD technology utilizes type IIB restriction enzymes to generate an isometric 33-36 bp restriction cleavage by genomic digestion. These tags are enriched for downstream high-throughput sequencing reactions and analyzed by bioinformatics, hence achieving genome-wide high-throughput SNP genotyping.”
Mike also shared with us the technical flow of 2b-RAD during our interview. He said it can be generally divided into four steps including Enzymatic cleavage of genomic DNA using type II restriction enzymes; Recover specific length (33-35 bp) fragments; connection connector; PCR amplification using sample-specific primers and sequencing of amplified products.
“Compared with RAD technique, the 2b-RAD has wined more popularity among clients because of its advantages”, said the director of sale in CD Genomics, Judy Clark, “our 2b-RAN is complete library sequencing, no loss of restriction loci, with ultra-high-density coverage of the genome and more complete information. In addition, the process is simple and the technical repeatability is good: the result of one sample is highly repeatable. Another merit means its uniform tag sequencing depth, which ensures the reliability and accuracy of each tag.”
Now, the 2b-RAD technique from CD Genomics has already served several institutions. Mike also expressed they would continue to improve this technique and where there is a need for clients, there would be qualified service from CD.
About CD Genomics
CD Genomics is the Genomics services company specializing in the services of DNA sequencing, genotyping and DNA library construction. With both traditional and modern genotyping platforms like Illumina BeadStation 500, Beadxpress and Sequenom, CD Genomics can provide services in each and every area of the genotyping field with a focus on pharmacogenomics.