The RapidFire High-throughput mass spectrometry system (RF-MS/MS) facilitates drug discovery programs for challenging targets, which are difficult using conventional approaches. It helps in the label-free screening of substrate and products, which are otherwise too small to label for intractable targets.

The mass detector removes the requirement of cumbersome labeling methods and surrogate substrates, which may not represent native reaction accurately, and further RapidFire technology help in high throughput assays processed at speeds matching with traditional plate-based optical methods.

1. Biosys optimized RF-MS/MS methods for multiple analytes in a single injection, completing 96-well plate sample analysis within 21 minutes.
2. RF MS/MS enables label-free screening through the detection of analytes that are otherwise intractable.
3. Major applications in cellular and biochemical assays in early drug discovery.
4. RF technology designed to enable high throughput assays at speeds matching traditional plate-based optical methods.
5. Accelerates SAR optimization by rapid generation of data from a focused library of compounds in only days.
6. Accelerates typical ADME experiments viz., CYP inhibition, permeability assays, plasma protein binding & solubility.

How RapidFire application saves time with high accuracy:

For an integrated discovery project, Biosys optimized the RF-MS/MS method in 96-well plate format to ensure analysis within a span of 21 minutes resulting in the per-sample run time of around 14 seconds.

RapidFire finds its major application in cellular and biochemical assays during the early-stage drug discovery process. The fast analysis of in-vitro screening of hundreds of library compounds in only a few days is highly useful in accelerating SAR optimization in drug discovery otherwise taking months together on traditional LC-MS/MS methods. We have demonstrated that RapidFire can be very useful in screening extremely high-value targets with high data quality.

RapidFire also exemplifies its application in typical ADME (Absorption, Distribution, Metabolism, and Elimination) experiments viz., CYP inhibition, permeability assays, plasma protein binding, solubility, etc.

For any further information on how Jubilant Biosys can help accelerate your discovery programs, please contact https://www.jubilantbiosys.com/